LOCUS JO316629 694 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c57888_c mRNA sequence. ACCESSION JO316629 VERSION JO316629.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 694) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 694) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..694 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 179 a 182 c 161 g 167 t ORIGIN 1 aacaagaaga aytagtgcgg ccattsccgc trcaaagaaa gaagtcttca tggtggcaga 61 ctgctgtgga caacagccaa agtggcttct ggtggtggta ggggctactc cttcctcgct 121 ggtgaggaat ccacgaccca ataacagggc ttataataat cggaggcatt cattscccgc 181 cccctgtttc tggaggagga gcttcaatgc cccgcaagag ccaaaggcga tcttgctgtg 241 tccatttgct ttccaacagc ctcgattttc aatccaatct catggagctt gatgtttgag 301 gtgtcacgat gatggagata acctccagca tgagatgatg caactgcttg acaagacctg 361 tttcctcttc ttaatgccag gtcgggtccc cgggacctcg actacgcaat gcgcccgtta 421 agttaaatgg aattcaagtg aagccgtgcr ctacgttaag ctagatcaat gagtcggtgt 481 agaattttga tagcgatcca gctttcaact ctcacagtct ggcgccggtc cagacgcccc 541 cttcacagtg cacacactga taccaaggaa cactgccata ccagcccaaa cccatccaat 601 ccaaaacaat caaactgatg caagacgaat gattgactgc gaattgctgc tcgtgctgag 661 agtcgatctt ccgcctttcc aatttggaaa cttt //