LOCUS JO316566 523 bp mRNA linear TSA 11-JAN-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c57227_c mRNA sequence. ACCESSION JO316566 VERSION JO316566.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 523) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 523) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..523 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 113 a 139 c 157 g 114 t ORIGIN 1 cggacggact tgaggcgaga aagctatggc ggcaatggcg acggcttcga ccacatttac 61 cagccgctgt gctggcggcc aattagctgc tgcgacttcc gggcttgcaa cgggatctca 121 gacgaggcag caacaacagc aaggcattgt cttctggtcg cggaacttga acgagtaccc 181 cagcacgtcg atccaggccg tccaatgcgg atcatccgct gggtcggagg tgttcggtaa 241 ctcatccatg gctggattga ggcaaccaag cttgaaggcc gtgatgacgc gtggcactgc 301 atcgaccggc attgtgtgca aggtgtccaa gggtgacgtg gcaccaccac tttctctcaa 361 ggatcagaat ggcaaagttg tcaacttgtc gaagtttagg ggcaagaacg ttgtcctcta 421 cttctatccc gcagacgaca cccctggatg cactaaggag gcatgtgctt tccgtgatgc 481 ctatcaggat ttccgcaggg ccggtgccgt ggtcattggt att //