LOCUS JO316485 531 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c56552_c mRNA sequence. ACCESSION JO316485 VERSION JO316485.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 531) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 531) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..531 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 125 a 153 c 126 g 123 t ORIGIN 1 cccctcgccg ccatcgccaa cgccgttcga gccgtgcgct gtggcacgcc tcgcactact 61 ttctcatgat cctgctaggc gccgacatgc cttgaatcaa tcttgcacac aagtttctcg 121 tctcgaatct cgatagacya rttctagatc gcgtaaggag cgtagcagag actggaaaat 181 acggtcaaaa taggaggaga aggtgccgga aagaatgacg ttggactttc tctcgctgat 241 gatgcactgc tgcaaagaca gaggagtacg ccaacgtttt gcgtcgtaag atttgaaact 301 tgaggcaatc caacccgacg aagatcgatc gagactgcaa tcagctccac gcttcgctgt 361 cagattccag gttctaaatc cctgaagcca ccaatcaaga gtccggcatt cgacagtacg 421 ctgagttgca cccgatgatc ctcctcgctc ttctccctcg tcgatatggt yccgsgaacg 481 ctaagatgca cccgatgatc ctcctcgctc ttctccgtgt cgatatggtt g //