LOCUS JO316055 386 bp mRNA linear TSA 11-JAN-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c54124_c mRNA sequence. ACCESSION JO316055 VERSION JO316055.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 386) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 386) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..386 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 101 a 75 c 107 g 103 t ORIGIN 1 tgcggcagcc atcgccaact tgcttgaagt ttttgttttg tccgtttcga agagcgagag 61 acaagatgcc gaccgattgc tgtacctgca atccttgcaa gtgcaccaac tgtcagtgct 121 cgaaaggtgc aaagtaactg gtgtggaatg gtaaaccgga cagtcggagg ggcgactgct 181 ccgattggtt gaacttgacg gtcatcatgg cgactagcca tgatgacaag ggggaaggaa 241 tcgagaggag attaacaaga ggttgttcta gatgtccatg cgtgcggcag ataacttttt 301 tcgtgtatgt catttttgga catttgttct gccgatatca gaaatcatga aaacattatg 361 tccagtcgtg aaaaagcctt gattag //