LOCUS JO315811 436 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c53289_c mRNA sequence. ACCESSION JO315811 VERSION JO315811.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 436) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 436) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..436 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 99 a 71 c 141 g 124 t ORIGIN 1 ccgagcggtc gacctgggat tttcgttttc ttagaatgga gaaggggtat ggtattctag 61 ggcacttgtg aatccatggt tacggattga agcgtgtgcg cgttattgat gattatagtg 121 cgcgcagaat tgatgaagcc tgctgcgcta ttgatgattg cagtgcgcgc agaattgatg 181 aagcctgctg cgctattgat gattgcagtg cgcgcagaat tgatgaagtg tgcgtgcgct 241 attgaaggtt acggtgcgtg cagtattgat gaagtgtggc tattctactt catggagtat 301 tgtttttggg aggtatgggg attcttcgac agtagcgaca tggagcggat tgaagtcctg 361 gaacgtaaat cgctggaacg aagacacagt gtgccctatt ggaagaaagc tgtcatgcca 421 tgcaggtgct ggnatg //