LOCUS JO315650 840 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c52822_c mRNA sequence. ACCESSION JO315650 VERSION JO315650.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 840) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 840) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..840 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 199 a 255 c 150 g 236 t ORIGIN 1 ctgcacaagc taccaaccgt ggaatctggt gatggacgct catgcagccg catcgcccac 61 tattctagct cctttcaacg ggttgccaac ccatttcatc agtagggaca ctcctagacc 121 ataatcctac tcaaaacttc tattttgtac aaactaccaa ccatagagtc tggtaatgca 181 cgcttatgcc gtcgcatccc tcgcacttct aactacttcc taaggcacat ttgcctgtcg 241 ctcattactc tttggttgtt tgccaagctg tgaagtaaga tgatccgaaa aataatatgc 301 aactcgaaaa atccacaata tcactcgtag tggttcatgc tcgtgaatct ctaggaagta 361 gaaatatccg aaacaataat atgcaacttg aataatccat ctgcacatta taccaccaaa 421 tccatcctct ccgcctaatt ggtcctctcg ttttgttctc cccgtgccta tcattttgtt 481 ctgcccaatc gtgccagttg atggttgcgt gcctcccgtt ttcctctgcc caaccatgcc 541 cgtcgtttcc tcctgccctt atcacccctt gcgcctgtga tgcggcactg aatgtgccat 601 cttgcacgtc acctgtcctc ttaattacgg agtttcttgc agccgcgtgg cacacaggcc 661 tcaagcaatc gaggctagtt tgtcctcaca gcactacgag ggatatcacc accaccacca 721 ccactgcttc tgttggtatc ccgatggttg ttgcaggcca cttgcttatg gatgtcccaa 781 agcacaatct ctctgtcatt tgaactcgtc aaaaccaaca tcgatccatc ctgaatcccg //