LOCUS JO315434 388 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c52236_c mRNA sequence. ACCESSION JO315434 VERSION JO315434.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 388) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 388) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..388 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 122 a 95 c 71 g 100 t ORIGIN 1 atatcttgta atgcgccacg tcccttagat aaattagctg cgcaaccacc aagtaataac 61 aatggacttt cagccatttg agcatttttt attgcagtta ccgtattagt tagtccagga 121 ccagcggtga caacacaaac accaatttta ccacttagac gtgagacggc atcagcagca 181 aatgcagcat ttgcttcatg acgagtatca atgatacgta taccacgctt ctcacacgca 241 accaatattg gtgatatatg accaccggat aaacaaaaca ggtagggaat tccatgagct 301 tttaaaacat cagccacaag ttcaccaccg tatgccgtcg attcttcgtc aactttgtag 361 acaaaagtgt atatccaacg tgtcccgg //