LOCUS JO315019 263 bp mRNA linear TSA 11-JAN-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c51177_c mRNA sequence. ACCESSION JO315019 VERSION JO315019.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 263) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 263) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..263 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 87 a 67 c 51 g 58 t ORIGIN 1 aagatttcac tcaagagata atacatgaat ggtggtggaa acaaaaaatt aggaaatcca 61 aaaataaccg aggggtgggg gggaaaaaaa aggaaaggga aatggtttat ctgtcgctct 121 ctcttatttt tactaatctg gcccttaatg gaacacctcc cctctccata caaacactca 181 aacctcctgc acgtgcacac acacgtgact ccgccagcta tatatgccac acccaatccc 241 caaccggggt tgcaagctac ctt //