LOCUS JO313446 489 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c48013_c mRNA sequence. ACCESSION JO313446 VERSION JO313446.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 489) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 489) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..489 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 128 a 112 c 121 g 128 t ORIGIN 1 tctctatgat caacgatgcg gaaaacatgc tgcgcacttg aattcccggg aacgtatcaa 61 agtcattggt ttatcataga gacgatttcg ggcaaaagaa attcccatgg tgtgacaccc 121 gtgcatcttg gattggaaat ccgacgtgta gaatgagtcc tctacatgaa aacttagttc 181 acactgcagt acagaaagtc tgactttcgt gccatttcag gtgccgtttc ttctacggcg 241 aatttgcccc ctccatccca tgtggcgacg ttcgtgcccc tttgtcagca tggttgagag 301 tgatgctgcc aactttcacc gagtttggtc ttgcaaagaa cacggtgcac tggcaaaata 361 cagcagtatg tgtgactgag caagcacagc cgcaggatat tttgatccga gagcaaccag 421 ttgccggaat tcccctaaag gcagtggaac ggtcagtgta atggaatgag gcctttataa 481 gttatgatg //