LOCUS JO311576 618 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c44706_c mRNA sequence. ACCESSION JO311576 VERSION JO311576.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 618) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 618) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..618 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 165 a 117 c 134 g 185 t ORIGIN 1 tcaagggtta tccccccatg ttttgaaaag ttaaaatttc acgggggagt tgcaaagttt 61 agcagtgcct gcatcgagac ctctgaggct taaactgcag gtyacaaaac acagcscwct 121 scacagtgaa atgcatgcgt ttcactcttc acgagtgatg tttctcacca ggaagtcggg 181 gtacttaggt ttaagataaa tctttttgac accgcaaggg tggatggctr gagattgtga 241 gcagaaaatc tagttcttga accttgcgtt aaattttgtw ttcygttayc ttttgaaaag 301 ccttyttcat ttcaaatatc tttatgacak cattagcggg aaaaagggtc tttttgatkt 361 caattcctga tccytgggat aggttgctgt tgtagtcytc aacccaaatg ttgttgatat 421 yttggacaaa tgggaaggta sgcccaktag grgtaccgtc tccgagagac aaaatagttc 481 aagagggtat gaaagccttg cttcgaacct tcttcttaat accagccacg gatttctttc 541 gaaatattgc tctgtattct tgggcaatct ttgaagatgg cgatgcaaaa acctccaaca 601 gcgcttttgg aacattta //