LOCUS       JO311554                 275 bp    mRNA    linear   TSA 23-FEB-2012
DEFINITION  TSA: Nitella hyalina strain KGK0190 nhya_c44670_c mRNA sequence.
ACCESSION   JO311554
VERSION     JO311554.1
DBLINK      BioProject: PRJNA51163
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Nitella hyalina
  ORGANISM  Nitella hyalina
            Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales;
            Characeae; Nitella.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F.
  TITLE     Broad phylogenomic sampling and the sister lineage of land plants
  JOURNAL   PLoS ONE 7 (1), E29696 (2012)
   PUBMED   22253761
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  AUTHORS   Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2011) Cell Biology and Molecular Genetics,
            University of Maryland, Bioscience Research Building, College Park,
            MD 20947, USA
COMMENT     Two-step clustering process: First, trimmed 454 reads were
            clustered with MIRA v.2.9.43 using 454 EST assembly specific
            parameters. Second, the raw Sanger reads were combined with the 454
            contigs + singleons (with quality scores) and were cleaned then
            clustered with CAP3 (100 bp plus 95 percent identity overlap).
            
            ##Assembly-Data-START##
            Assembly Method       :: MIRA v. 2.9.43; CAP3
            Sequencing Technology :: 454 GS FLX Titanium; Sanger
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Nitella hyalina"
                     /mol_type="mRNA"
                     /strain="KGK0190"
                     /db_xref="taxon:181804"
BASE COUNT           76 a           45 c           56 g           98 t
ORIGIN      
        1 tctatgtcag ctacttcgcc gtacggtaga gaaaaatcac ccatggatgg aaattttaac
       61 tatagatgga ttagtccaca cacaaggtcc ttttaaacat agttggtggc gaatattttt
      121 gattatattt gctgttcttg cgtttattct tacgtgcatt ttcaatggat tagcgagtta
      181 caggtttcaa atggaatatt tattcaacga actggaagtg tgagtgatga caatcttact
      241 gactttactc ctgcaggttg gacattttgc aattt
//