LOCUS JO311520 376 bp mRNA linear TSA 11-JAN-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c44597_c mRNA sequence. ACCESSION JO311520 VERSION JO311520.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 376) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 376) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..376 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 88 a 51 c 70 g 167 t ORIGIN 1 ttattttcaa taaaagaaaa caataagttc ttagtaaacg tgttactact agttgttgat 61 tcgattcagc tagatgtttg tcagcttgaa ccgtatttgt ctgtttctgc atggcgagaa 121 tgttttgtaa tcgttttgat tcgttggttg ataactgata tttcaatatt attcggattt 181 ggatgaaatt ctttcatttt ctgtttgtgc ttttcttgct gttcttctaa tttttttcag 241 tagttctttt gccatgaatc ttggaattat tctctgaata gatcttcttc atcttcttcg 301 ttaatcattt ggtatagacc atcgctgaat gggatcgttt gttgtcgtag atgatttgta 361 ttgatcaaga attgag //