LOCUS JO311399 495 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c44401_c mRNA sequence. ACCESSION JO311399 VERSION JO311399.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 495) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 495) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..495 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 152 a 101 c 90 g 152 t ORIGIN 1 tttttggttg acaacatatg gatgattgga ggaaggccat caaccaatgg aaggccaatc 61 caagtaacct cgaccttcgt tatatgaacc tcgggaagaa ggagctcttt atcttgcaaa 121 cgttttgaag tcgactgatc tttctactaa agcttttggt gactttgaaa gtttttcaat 181 ttcaagtaat gatgttggtg ataaagggct tatgtatatt attgatggga ttcttcatag 241 ccctttcact agattaaaaa acatttgacg tatctttcaa caattgcact caatgttgtg 301 gagcctccct catcaggctt cttgaaaact cgaacatcac agagcctttt tttgtgggaa 361 atgaaatgga tccaagcact gaagcacaaa tttttcgttt gattgaacaa aacaaataac 421 aaaaagactt ccagaatttt caccagtccc acttctgcca gtataactgc acctgcctct 481 ccaaacactc aagat //