LOCUS JO309904 413 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c41974_c mRNA sequence. ACCESSION JO309904 VERSION JO309904.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 413) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 413) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..413 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 133 a 57 c 95 g 128 t ORIGIN 1 aagaagggag aactaattat gggaattgga cacagagtaa aaactcttca gaatcccgat 61 aagagagtta ctattcttaa agagtttgca ttggctaact ttccttctac aagtgtaatc 121 aaatatgctc ttgaggtaga gaaggttaca acaacaaaaa gaaacacatt gattcttaat 181 gtggatggat gtgttggatg ttgttttgtg gatttacttc gaggatgtgg agctttcaca 241 aaaagaggaa gcggataact acatcacact tggatttttg aatggaatgt ttgttcttgg 301 aagaagtatt gggctcattg gtcactattt agatcaagta agattaaagc agcccttata 361 cagacatcca tgggatgata tttcttatgc tgatgatact gctctgatgt gag //