LOCUS JO309787 490 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c41801_c mRNA sequence. ACCESSION JO309787 VERSION JO309787.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 490) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 490) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..490 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 110 a 133 c 126 g 121 t ORIGIN 1 cgagctatgg acgccacctt tcaaggcttt tgttaatcga tggagctttc aaaacctgcg 61 ctcgagtttc atcggcacaa aggtgggtac tttccgcagt ttcccgggaa tgcaagtgct 121 atcgaaggat gacacgtgtc cattctacga tccccgctac agatcgtggt tccagaaggc 181 ttcgtctcca cgtaagaacg ttatgattgt tcaagcagca ggatcagata tggcggaacc 241 tatgttatcg ttgtcatcgg gccggtcacg actgagcatt tcctcggaca ccatcgccga 301 gcttctcgga acatttcgag agtacgatcg agtcggatgg gtctcgtatc ccagcaacgc 361 ctcctttcct ctgacaccag cagggcaggt agttgcaggt gcaaagcttt ctgatgagct 421 taagccgctg gagacgcggg ttcgaaccct gacgccgtca tcttcatccg tatccaactc 481 aagcgcaatg //