LOCUS       JO309283                 519 bp    mRNA    linear   TSA 11-JAN-2012
DEFINITION  TSA: Nitella hyalina strain KGK0190 nhya_c40991_c mRNA sequence.
ACCESSION   JO309283
VERSION     JO309283.1
DBLINK      BioProject: PRJNA51163
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Nitella hyalina
  ORGANISM  Nitella hyalina
            Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales;
            Characeae; Nitella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F.
  TITLE     Broad phylogenomic sampling and the sister lineage of land plants
  JOURNAL   PLoS ONE 7 (1), E29696 (2012)
   PUBMED   22253761
REFERENCE   2  (bases 1 to 519)
  AUTHORS   Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2011) Cell Biology and Molecular Genetics,
            University of Maryland, Bioscience Research Building, College Park,
            MD 20947, USA
COMMENT     Two-step clustering process: First, trimmed 454 reads were
            clustered with MIRA v.2.9.43 using 454 EST assembly specific
            parameters. Second, the raw Sanger reads were combined with the 454
            contigs + singleons (with quality scores) and were cleaned then
            clustered with CAP3 (100 bp plus 95 percent identity overlap).
            
            ##Assembly-Data-START##
            Assembly Method       :: MIRA v. 2.9.43; CAP3
            Sequencing Technology :: 454 GS FLX Titanium; Sanger
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Nitella hyalina"
                     /mol_type="mRNA"
                     /strain="KGK0190"
                     /db_xref="taxon:181804"
BASE COUNT          110 a          150 c          102 g          157 t
ORIGIN      
        1 ttcttaaaac cggaccttta aggtcccttt aaacgcgtcg ctctttccat aaaacccctc
       61 cagtaatttg cacagcttgg gccactttat ccataaaacc ccttgtacta ctttgtatgg
      121 attctttatg ggaaacttga atttatttac tttgtaccga atcattcatg tctgaaaggt
      181 gtgcagtgtg tgccacttgg gtggggctgg caggatgtgt gctccgcttg tgcgatgtgt
      241 gcaggatgtg tgtggcgatt gttcatcatc cccacggttt tcatcagcct ctcgctaaaa
      301 ccccccttat cgccatgatc cctcgtccta cacacccctc ctcacacgcc ccgtaactcg
      361 ctgtcctttc cctgttgtgc ccaatccttt ttgcgagctg ttgaaaagaa tgtgggttca
      421 atttttgtca aaccaaccat tagcaaccac ctttcccgct ctcaagccca aggccgtgac
      481 aagctctcga gcaacccttg cgatctatcc gatgactat
//