LOCUS JO308175 401 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c39239_c mRNA sequence. ACCESSION JO308175 VERSION JO308175.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 401) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 401) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..401 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 103 a 67 c 113 g 118 t ORIGIN 1 tcctagttat attcgtgact catcagttgc tgttgttgtc tacgatgttg ccaaccgaca 61 atcatttgtg aacacggctc ggtgggttga agaagtaagg acagagcgtg gcagtgatgt 121 gatcattatg cttgttggaa acaagacgga ccttgtagag aagagacaag tgtcgattga 181 ggaggcgtgc ttgactatta taggacggtt gattgaaacg tcttgaaatc gagagtgata 241 gcctaggttt gtttcttcta atagccgttg cttctagtag gcttgagatg atttcgagca 301 ccaaccagat cggtattgcg accgaggggg aaatgagagg gactcggagt gggatctgaa 361 ctgtgtaatt attgtaccat acacttcaat gagttttctc t //