LOCUS JO305691 426 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c35388_c mRNA sequence. ACCESSION JO305691 VERSION JO305691.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 426) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 426) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..426 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 109 a 105 c 89 g 123 t ORIGIN 1 agcacggcat cttccatcat ttgagaccac gacaagtgaa ccattcagtt gctttttgac 61 atccccagga accatcacaa tcaggattga tacggcaatt gggttggtta gggagatctt 121 tacacgaaag tataccttgc tcaaagcagg cttcggcttc ctccttttct tggcgtgttg 181 gaatgtagtt tctatcgagg aattttatcc acaattgctt ggtcacgaca gatagcatag 241 cgataaatgc ctccatggtc tccaaaatcg tcaatgcaga attcagtttc gacgatctcg 301 ccaggacgaa aattggccac aagctttccc cagtcattcg acgggctgtt gtagtcaaga 361 ccaccaaagc tctttccaca cggtccaatg tattcgttgt ttcctagttc ggattagctt 421 aactct //