LOCUS JO305000 431 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c34297_c mRNA sequence. ACCESSION JO305000 VERSION JO305000.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 431) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 431) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..431 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 91 a 128 c 75 g 137 t ORIGIN 1 tttgtacaaa gatgcgtaat ggataaggct gtctacattg tgtggatgcg atgtcaatta 61 acttgctcgc tctctcgctt gggcacacac actctttccc gctcttcttc cctccatttc 121 ctctctattt ccctccctcc attgcctcac accttcttca ctcctttcca aatgttcccg 181 ccaaaatctc ttccgttttg taatcctctc ttccttatcc ccgcacagac gcacattgtc 241 ttgatcatcg ccatgtcgga ttcgtctccg ccagctcagc aacaactgca ctgctgagtc 301 agcgcgagga attatctcgg tagattgaat gggaacctac gctgcattgc gttacataag 361 catttgttcg cgcccttttg tattttcact caacctaaag aagacgcgat tcatctggtt 421 gagtgaaaaa t //