LOCUS JO304719 538 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c33846_c mRNA sequence. ACCESSION JO304719 VERSION JO304719.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 538) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 538) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..538 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 117 a 165 c 96 g 151 t ORIGIN 1 tcccctacct ctccttcctt tccttcctcc tctcctcgca cacacctcct ctctctctct 61 cgctctctct tcatttccaa acacaacaca cgyttttccc tgattacggg tcaatgtatt 121 agacgattat gctggacgat sscacttctc aaggtatgaa gattctgtca cctggrtaga 181 ttctrtcagg atcgtcrgta acgtcagtgt ttgcctcgta taagagatcg gctgtaagat 241 tataacaccc cgcgatggca cagagagtrt cyccttcctg aacctcataa tacctttgct 301 gtctctgtcc ccttcccaca gccaatgawg tcatcagaac tgccacaagt gctccaatga 361 tcgagaagaa tcgactgcgc ctattgccct tgctccctgc cttcccctga gcagctaacg 421 tgtcttggct gtcagaaaga tcaggttttt gaaaatcgaa accctcaggt gatgcagcca 481 gttctgcagc acgcaatgct ttgccatctt cataaaccag cttctcagga ttcccggg //