LOCUS JO304469 438 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c33447_c mRNA sequence. ACCESSION JO304469 VERSION JO304469.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 438) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 438) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..438 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 122 a 88 c 101 g 127 t ORIGIN 1 gtcacaacag accttttcag gtattatcga gagtgtattt ggactaaact gctgtccata 61 tacattgcaa atcataccag ataaggtctt gagatgcatc agattttgcg gtgctgcatg 121 catgtggcca ctgctacagt cttcccccag atatcattcc taggtgttat acttgaaaag 181 cagccgaaat tcaatcatag cagcttggct tctctgaata tactgcctgg gcgttacatt 241 cgggggaaga ctgtaactat gagtgacatt gagtggctat cgctgacaat gactgactat 301 aagtgattgt gactgactct gactgactgt gattaactat gatttattat gataggctgt 361 agctcgctat gactggcgat aactggcaag atgaaaagac aaagagcagt gttgcattca 421 ccgaacagtg ttcgccat //