LOCUS JO303266 284 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c31484_c mRNA sequence. ACCESSION JO303266 VERSION JO303266.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 284) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 284) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..284 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 73 a 47 c 52 g 112 t ORIGIN 1 atcggacatt ctaataaaaa actgtgaagt attggcagca atttcagtga gaatcttatt 61 ttgatccttc agcttcttgt catgataatg taatatgttt gcttgcaagt ttaaagattt 121 ttctcttagt cttaattgta actcttgtcg gacttgtatc ttttgaagtg atacccgcag 181 gtttacactt tctttgtcga gattttcgca tgatttcttt aaaataaaga gatcttggtt 241 ttggaattga aacggagtgg atccttgcct cgatttctca tccc //