LOCUS JO290536 566 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c13299_c mRNA sequence. ACCESSION JO290536 VERSION JO290536.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 566) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 566) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..566 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 149 a 111 c 153 g 153 t ORIGIN 1 cgggttggat acacgtggaa agcccacggg gttggataca cggaaagccc acgggttgga 61 tacacgtgga aagccactgg ttggatacac gtggaaagcc cacgggttgg atacacgtgg 121 aaagcccacg ggttggatac acgtggaaag cgcatgggtt ggatacacgt ggaaagccca 181 cgggttggat acacgtggaa agcccacggg tgcttgtcga tctgctgagt cttgttcaca 241 tttaacgatg atgtgacgaa cctcacggtg ccacgttttg ggctctctgc tacagccaca 301 ttggctaaac gtttgtccgt gtttggtagg agttgatgga tcagacagtg tctaattacg 361 tacgtgcttt atgtgctcgg tgaacgcacg aataccttag attccgggtc atgtagatca 421 tatccagtga ttttaaactg actcatatct gtaattaaaa accttacgaa tactttagat 481 tccgattcat gattagatta tgtagtgatt gcaaactgac tcgaagttgt aattagaaaa 541 tttgtgtaat ttacatgcag cgattg //