LOCUS JO289554 462 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c11991_c mRNA sequence. ACCESSION JO289554 VERSION JO289554.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 462) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 462) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..462 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 125 a 97 c 135 g 105 t ORIGIN 1 cgggatagag cacgaggtta ttcccggagt cgtagaaagt ttgaaggtga tcaccaaaca 61 taactcggag cgcattgcga agtacgcatt tgagtatgca tatctgaatg aaagaaaggc 121 tgtaacagca gttcacaagg ccaacatcat gaagcttgct gatggactct tcctagagtc 181 gtgccgacaa gtggcaaaac aatacgaggg gattcgctac aacgaggtca ttgttgacaa 241 ctgttgcatg cagcttgtgg ctcgccctga gcaattcgat gttatggtga cacccaattt 301 gtatggaacc ttggttgcaa acacagctgc ggggattgct ggtgggacct cagtgatgcc 361 tgggggcaat gtgggtctgg agcatgccat cttcgagcaa ggtgcgtcag cagggaacgt 421 tggcaacgat tggctggtta agaataaagc cgcgaatcca ac //