LOCUS JO271009 460 bp mRNA linear TSA 11-JAN-2012 DEFINITION TSA: Klebsormidium flaccidum strain UTEX 321 kfla_c35388_c mRNA sequence. ACCESSION JO271009 VERSION JO271009.1 DBLINK BioProject: PRJNA51159 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Klebsormidium flaccidum ORGANISM Klebsormidium flaccidum Eukaryota; Viridiplantae; Streptophyta; Klebsormidiophyceae; Klebsormidiales; Klebsormidiaceae; Klebsormidium. REFERENCE 1 (bases 1 to 460) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 460) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..460 /organism="Klebsormidium flaccidum" /mol_type="mRNA" /strain="UTEX 321" /culture_collection="UTEX:321" /db_xref="taxon:3175" BASE COUNT 89 a 143 c 146 g 77 t ORIGIN 1 attcccggga ggcagggttc ggacaagtcg ggctggcatt gagcttcatg attggatttt 61 tcctgttcac ccgcttctgg aagcccgccc tggccttcat caccaacgtc atcttcatca 121 tcttctcctt cgggcgctac cgccccggtc aaaaacagcg cctggcccgc gagcgcgcgc 181 gggccgctct agttgccacg ggaccgatca ccctcgacct cgacgacgaa gccgcccccc 241 agaaggaaga gatcattgac attgctctgc ccccggaaat agagctggac ttggacgctg 301 aaagcacaag cgggcggacg gagtcgagcg ccgtccgtgc gaaakyscck sgatgtgaat 361 gcggccacct agtgtgaacg cgcccgagcc tagtggggag gcagctgcgc cggagggtgt 421 gaagaaggcc aagggggcgc tgcgagaggc gccgacgctg //