LOCUS JO270728 550 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Klebsormidium flaccidum strain UTEX 321 kfla_c34339_c mRNA sequence. ACCESSION JO270728 VERSION JO270728.1 DBLINK BioProject: PRJNA51159 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Klebsormidium flaccidum ORGANISM Klebsormidium flaccidum Eukaryota; Viridiplantae; Streptophyta; Klebsormidiophyceae; Klebsormidiales; Klebsormidiaceae; Klebsormidium. REFERENCE 1 (bases 1 to 550) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 550) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..550 /organism="Klebsormidium flaccidum" /mol_type="mRNA" /strain="UTEX 321" /culture_collection="UTEX:321" /db_xref="taxon:3175" BASE COUNT 101 a 174 c 157 g 118 t ORIGIN 1 cagcagggtc aactccgact ttagcggcga aagtcctgaa cgtccccagg atcggcaatc 61 ctctgcggtg cgcctccgac cgcttcatca gcagaactgc cgccgcgccg ttcggtgatc 121 tggctcgagt tgccggcggt tgtggatccg tcctttttga acacggcctt cagcttggcc 181 agactggcgg gggtgacgcc ctcccgcatg ccgtcgtccc gtgagatggt gacgggcttc 241 tcgtcaccgg tcttcggtcg acgatcttcg tcgccaccgg aataacctcg tccacaaatc 301 tgccggactt ccaagctgcc accgcgcgag catgagaacg agcggccatc tggtcttggt 361 ctgctctggt gatgccgtac cgctcagaaa ggccctgggc cactccgatc tcttctactt 421 agatgataaa gagcttgatt tcagcgagat tattgatgca ccgctcccaa agccgccgga 481 cttgacacaa cggtcattgc acactggctg gctgtggatg gggtggcagc cagccataca 541 ggagaaccct //