LOCUS JO270419 491 bp mRNA linear TSA 11-JAN-2012 DEFINITION TSA: Klebsormidium flaccidum strain UTEX 321 kfla_c33447_c mRNA sequence. ACCESSION JO270419 VERSION JO270419.1 DBLINK BioProject: PRJNA51159 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Klebsormidium flaccidum ORGANISM Klebsormidium flaccidum Eukaryota; Viridiplantae; Streptophyta; Klebsormidiophyceae; Klebsormidiales; Klebsormidiaceae; Klebsormidium. REFERENCE 1 (bases 1 to 491) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 491) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..491 /organism="Klebsormidium flaccidum" /mol_type="mRNA" /strain="UTEX 321" /culture_collection="UTEX:321" /db_xref="taxon:3175" BASE COUNT 119 a 145 c 114 g 113 t ORIGIN 1 cggctcagac aacactttcg agaagccttt gaaatcccta ttcgacctaa tcgggtttgc 61 ccaaaccgcc gtggacggat gtgatgtcca ccaagaggaa aaccagctac ggatctgact 121 tcgtagacaa cacagacggc ttagacaacg cattcgtaga agaccttcga atttcggatt 181 cggcatgatc actcaccagg tgggtcgttt gatacgtgac gtaatctgca tacaacgccc 241 tgctcgtgat tgacgtctct cctgtcaggc gcttcaaatc agactccgag atttcgtcca 301 tctcacccgt ctctaagcac accgctatcc gggtgtctcg caaacgtgct tcatccggat 361 ttgcagcacc cttcccaaag cggcccgttg gcccttagac gtcaagagca tgtccttgga 421 gacatccttg tagaactgtc caaaggagat gcttcacaag acccaagagg aggctctttc 481 ccgatcccgg g //