LOCUS JO270416 310 bp mRNA linear TSA 11-JAN-2012 DEFINITION TSA: Klebsormidium flaccidum strain UTEX 321 kfla_c33433_c mRNA sequence. ACCESSION JO270416 VERSION JO270416.1 DBLINK BioProject: PRJNA51159 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Klebsormidium flaccidum ORGANISM Klebsormidium flaccidum Eukaryota; Viridiplantae; Streptophyta; Klebsormidiophyceae; Klebsormidiales; Klebsormidiaceae; Klebsormidium. REFERENCE 1 (bases 1 to 310) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 310) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..310 /organism="Klebsormidium flaccidum" /mol_type="mRNA" /strain="UTEX 321" /culture_collection="UTEX:321" /db_xref="taxon:3175" BASE COUNT 51 a 102 c 86 g 71 t ORIGIN 1 ggatcgtacc tggcgcttat gaccgtcatc tctactacct catgaacgac accgactctt 61 ctcggacgcg ttcggcgtcc gcaccctccg gcccgatccg aacagcgagc ccgtgagcac 121 gaggcgttac aaggagagag tggcggtcat ctacctgcag gtgtccatca tcagccaggc 181 actcatcttt gtgacccgat ccccagaggt tctcgtggct ggagcggccc gggttcctgc 241 tggtgttggc cttcctcgtc gcccagctga ttgcgacctt catcgccgtg tatgccacgt 301 ggaatttctg //