LOCUS JO270350 439 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Klebsormidium flaccidum strain UTEX 321 kfla_c33258_c mRNA sequence. ACCESSION JO270350 VERSION JO270350.1 DBLINK BioProject: PRJNA51159 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Klebsormidium flaccidum ORGANISM Klebsormidium flaccidum Eukaryota; Viridiplantae; Streptophyta; Klebsormidiophyceae; Klebsormidiales; Klebsormidiaceae; Klebsormidium. REFERENCE 1 (bases 1 to 439) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 439) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..439 /organism="Klebsormidium flaccidum" /mol_type="mRNA" /strain="UTEX 321" /culture_collection="UTEX:321" /db_xref="taxon:3175" BASE COUNT 92 a 106 c 173 g 66 t ORIGIN 1 acccgcagcc gcgtaaaccg gggaattcta caccaggaga aagaaaaagc ggggacaata 61 aagcgggcag tgctccaatc ggcgttccca tcggggccgt ttgggatgcc ggtcgggggc 121 gaacatggtg ttcggggtcc ggtagcacgg gagtttactg ctcagggggt tagggtttaa 181 cctgtccgct aaccnggtgt ttcccagaac tggcagggct cgaacatgaa cggaatggcg 241 gaggggatca aggcggggtt aagcctgagc gcgcttttgc agcagcagtt ggagttgcag 301 ttgcagcaga atcaggggcg ccgccggcgg aagggggtag gggggacaaa gaggaagtag 361 gggctatgcg nggcgccagc tggcggggcg gcagcaagga cttcgggctc ccagcgagcc 421 gcggcgcgcg cagcagccg //