LOCUS JO269862 347 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Klebsormidium flaccidum strain UTEX 321 kfla_c32017_c mRNA sequence. ACCESSION JO269862 VERSION JO269862.1 DBLINK BioProject: PRJNA51159 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Klebsormidium flaccidum ORGANISM Klebsormidium flaccidum Eukaryota; Viridiplantae; Streptophyta; Klebsormidiophyceae; Klebsormidiales; Klebsormidiaceae; Klebsormidium. REFERENCE 1 (bases 1 to 347) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 347) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..347 /organism="Klebsormidium flaccidum" /mol_type="mRNA" /strain="UTEX 321" /culture_collection="UTEX:321" /db_xref="taxon:3175" BASE COUNT 91 a 68 c 121 g 67 t ORIGIN 1 cgagcggagc agaagaggcg catgaagaga aaggcgaggt cgctgcaggg agggcggtga 61 cgtggacatg gcggaagcac cgagggagct ggaaggagcc atattatcct gaggcagagg 121 aggagcgtgc ctcagaaggt gcagggaaaa aggaggggac cgatggcgtc gtgcaaggat 181 gattggacat tttggggtac tccgattctc tgccggctct attatttgac cgggcgttca 241 caattatcga tgctgacaga tcctgtaaga gacgtcaatg aagagcatga gtggtcgcaa 301 gaggctgctt gatcggtcaa ttaaaatcgc gtctaatctg actacct //