LOCUS JO269857 252 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Klebsormidium flaccidum strain UTEX 321 kfla_c32006_c mRNA sequence. ACCESSION JO269857 VERSION JO269857.1 DBLINK BioProject: PRJNA51159 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Klebsormidium flaccidum ORGANISM Klebsormidium flaccidum Eukaryota; Viridiplantae; Streptophyta; Klebsormidiophyceae; Klebsormidiales; Klebsormidiaceae; Klebsormidium. REFERENCE 1 (bases 1 to 252) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 252) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..252 /organism="Klebsormidium flaccidum" /mol_type="mRNA" /strain="UTEX 321" /culture_collection="UTEX:321" /db_xref="taxon:3175" BASE COUNT 83 a 58 c 55 g 56 t ORIGIN 1 cgatttcaaa gaggaacact gggttcatgt gtccaaggaa gagcagacat cgtcaaaaga 61 tggacgtgac gtactgcagc tttttcctat ggagggttgc gagactctca gcagagtacc 121 aaaagtagta cccaacaagt gtatgtagat accgagacta ccagaaaaat accaaactct 181 gcttcgacag gatcgaagca gacttttccg ctaaccagct ttcatcaaac ccttaaatga 241 aagctcatga ct //