LOCUS JO269848 705 bp mRNA linear TSA 11-JAN-2012 DEFINITION TSA: Klebsormidium flaccidum strain UTEX 321 kfla_c31990_c mRNA sequence. ACCESSION JO269848 VERSION JO269848.1 DBLINK BioProject: PRJNA51159 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Klebsormidium flaccidum ORGANISM Klebsormidium flaccidum Eukaryota; Viridiplantae; Streptophyta; Klebsormidiophyceae; Klebsormidiales; Klebsormidiaceae; Klebsormidium. REFERENCE 1 (bases 1 to 705) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 705) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..705 /organism="Klebsormidium flaccidum" /mol_type="mRNA" /strain="UTEX 321" /culture_collection="UTEX:321" /db_xref="taxon:3175" BASE COUNT 184 a 176 c 163 g 182 t ORIGIN 1 aacctcaatg caaagggtac ttcaccgttt ttgctctcag tgaatccagt caaaaaactg 61 gatggcttcc tagctgttta aaaggtaagt tctttcgaga tttggaatga ttggagtgca 121 ctttacattg caacgatgaa gagaataggc agtcaagaga acatactacc aacgattata 181 tacatacccg gagtatgttc tcttgactta cattgcaacg atgaagagaa tggcagtcaa 241 gagaacatac tccaacgatt atatacatac ccggagtccc cagtcaagca ccatgatggc 301 gggttgagtg agctgtcgtt gattgcaacc gatattgtcc agactgtctc tgcctttacc 361 actgatgaaa acaatgcaac cgtcagctta ctcgtctgct gcgcttgctt gattgagatc 421 tttctcgctc aaatccagaa tcttccagta atcctggtat acatgctcag cacatcctcc 481 acagtggttt cgaagtgggt cgtcgggtcg tagctcttgg agatgtagca gttgtcgtga 541 ctcccgctct ccagcggctc gtacgtctcg taaatgtcga agatctgttc gtccacctgc 601 cccaacagct tcaagccggg accctagcta cttcccgggc aagattgcga agagtggaaa 661 ggtggtgcga gcaatctgca tcatgagcca cccggtcccg aacac //