LOCUS JO258531 438 bp mRNA linear TSA 11-JAN-2012 DEFINITION TSA: Klebsormidium flaccidum strain UTEX 321 kfla_c11408_c mRNA sequence. ACCESSION JO258531 VERSION JO258531.1 DBLINK BioProject: PRJNA51159 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Klebsormidium flaccidum ORGANISM Klebsormidium flaccidum Eukaryota; Viridiplantae; Streptophyta; Klebsormidiophyceae; Klebsormidiales; Klebsormidiaceae; Klebsormidium. REFERENCE 1 (bases 1 to 438) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 438) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..438 /organism="Klebsormidium flaccidum" /mol_type="mRNA" /strain="UTEX 321" /culture_collection="UTEX:321" /db_xref="taxon:3175" BASE COUNT 129 a 116 c 106 g 87 t ORIGIN 1 tgcggcaaga cactgcaagt gcagtccgat gcactcgcaa ccctttccga ctttgtgcac 61 agataggacc aagcacattg atattttact aacgtaaaac ccctggatgt gagtagccta 121 ttagccgcta cttgacatca agcaaagacg gttcaggatt actgaagcag ggcgggaacc 181 ttagccttca acgttacctt ctgcggtagt caggagatcg gctcctgcct cgtccatagg 241 ggcggtaatc cggcgacctg ctcctatacc gccggggtgg agagcggctg caaacagaat 301 gataaaacaa tctttctacg atcccaacta aaatgacgac aaaagggcat cagaaaaagg 361 tcaagcgaca ccactctatg acaaagactg agccactgca gaaggaaatc gcgacccatc 421 gacttggcaa agcagctt //