LOCUS JO232447 449 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Penium margaritaceum pmar_71836_c58799_c mRNA sequence. ACCESSION JO232447 VERSION JO232447.1 DBLINK BioProject: PRJNA68813 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Penium margaritaceum ORGANISM Penium margaritaceum Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae; Desmidiales; Peniaceae; Penium. REFERENCE 1 (bases 1 to 449) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 449) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..449 /organism="Penium margaritaceum" /mol_type="mRNA" /strain="SKD2004_CL18" /db_xref="taxon:102169" BASE COUNT 94 a 141 c 135 g 79 t ORIGIN 1 cttgctattt gcacttcgtc gcgggtcacg ttgcgcgaca ggaggcacca ccagcaccag 61 cagacaacac tccgcagcag cgccagcgac cgcagcagta gcggcagttg caacaccagc 121 agccgcacct ggaacagcag cagcagcagc catgcacgac ttctgtttca cgctgcctta 181 cggagtgctc ctcgcgctgg gcggcgtggt cggatttctg cgcaagggca gcgttgcgtc 241 gctggccggc ggcgtgggca gcggcctgct gctgtacctg gcgggccagt tgcagctcaa 301 ggccttcgag aaacggaaga actctcttcc ggcgctgctg cttcaacttg aggtgtgagc 361 gcgtgtgagc gtggtaatga cggtattcta cgtcgtcaaa atcgcaacag gaggcaacca 421 cttcaaacca aaaccaacct gaccagcac //