LOCUS JO232431 680 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Penium margaritaceum pmar_71836_c58376_c mRNA sequence. ACCESSION JO232431 VERSION JO232431.1 DBLINK BioProject: PRJNA68813 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Penium margaritaceum ORGANISM Penium margaritaceum Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae; Desmidiales; Peniaceae; Penium. REFERENCE 1 (bases 1 to 680) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 680) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..680 /organism="Penium margaritaceum" /mol_type="mRNA" /strain="SKD2004_CL18" /db_xref="taxon:102169" BASE COUNT 144 a 176 c 184 g 176 t ORIGIN 1 acgtcatgga tgtggatgct cactacggcg ggtttggcgc ggcccttgca gacaagccaa 61 tgtggagcat gaatgtggtt cctaccacca gccccgacac actgcccatt gtctttgacc 121 gtgggctgct gggcatctac cacgactggt gcgagtcgtt caacacgtac ccccgcacct 181 acgacctcct gcatgtcgat cacttcctcg gccacgctgc taagagcaag aggtgcgacg 241 tggcagacat catgctggaa tttgaccgca tcctccgccc tactggctat gtcatcatcc 301 gtgagcatgc tgatcgtgct cctgttgtgg aaggcattgc caagggcctg cactgggacc 361 tgaaaaagcg cttccaggag gagaatgagg ttctcctttc ctatcagaag accctctggc 421 ggccacagta ggggcctagg gagttacttt tccaggtagg gtaggtggtc agtgctttcc 481 cctccaatcc cacatgtgct gtgggtatga gctttcgata tttccttatt atatcataag 541 gcaggactca gccctaaaat tctttcccac ttctcttgaa ctgaagatgt ggatttggat 601 gtgggatgtt gtgtgctgtg ctgagagggg tcaaaactga cttttgacct gttcatatta 661 gtgagggaaa gagcttaata //