LOCUS JO232430 252 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Penium margaritaceum pmar_71836_c58339_c mRNA sequence. ACCESSION JO232430 VERSION JO232430.1 DBLINK BioProject: PRJNA68813 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Penium margaritaceum ORGANISM Penium margaritaceum Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae; Desmidiales; Peniaceae; Penium. REFERENCE 1 (bases 1 to 252) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 252) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..252 /organism="Penium margaritaceum" /mol_type="mRNA" /strain="SKD2004_CL18" /db_xref="taxon:102169" BASE COUNT 63 a 73 c 72 g 43 t ORIGIN 1 gaacaaggcg acgctggaac atgctgcaca agggtcgaac ccctasgtca cgtacggcta 61 ccccaacatg aagagcatcc cgcgagctca tctacaagcg cggctacggc aaggtggaca 121 agcagcgcat cgccctcact gacaatgcca tcgtcgagaa ggttcttgga aagcacggca 181 tcatctgtcg tgggaggatt ttcgatccca cgagattgtg agccgttggg gcccgcactt 241 tcaaggagga cc //