LOCUS JO232225 654 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Penium margaritaceum pmar_71836_c54917_c mRNA sequence. ACCESSION JO232225 VERSION JO232225.1 DBLINK BioProject: PRJNA68813 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Penium margaritaceum ORGANISM Penium margaritaceum Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae; Desmidiales; Peniaceae; Penium. REFERENCE 1 (bases 1 to 654) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 654) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..654 /organism="Penium margaritaceum" /mol_type="mRNA" /strain="SKD2004_CL18" /db_xref="taxon:102169" BASE COUNT 161 a 187 c 201 g 105 t ORIGIN 1 taagtacctc acgggtccat tgttgccgca acgtgtcgtg cctcattgtg tcgcacgact 61 cgtccttcct cgacaacgtg tgcactgaca tcatccacta cgacaagcgc aagctcagac 121 agtaccgcgg caacctcgct gccttcgtca aagtgaagcc ggaagcaagg gtctactacg 181 atctggaggc cgcggcgttt gcgttcaaat tcccgcagcc gggcatgctg gacggcatca 241 agcgcaagga gacgcccatc ctgaagatga ccaacgtcac gttccagtac tcgggcgcgg 301 agaagcccac gctggtggac gtgaatgtgc agtgctcgct gggaagcagg gtgggtgtgg 361 tcggtgccaa tggcgcgggc aagtcaacgc tcatcaagat gctgacggga gaaacgaggg 421 cctcgacggg tacaatatgg cggcacccca acctgggcat ggcgtacgtg gcacagcatg 481 ccttccacca cgtcgaacaa cacctggaga agagccccag cgagtacatc cgctggcgtt 541 tcgcacgcgg tgaagacaga gaagagctca acaaggtgca ccgcaagatc agcaaggagg 601 aggcaaaagg cgatggagga ccctgatcat gcacaacggc cagagaaagc aggt //