LOCUS JO230548 281 bp mRNA linear TSA 11-JAN-2012 DEFINITION TSA: Penium margaritaceum pmar_71836_c41032_c mRNA sequence. ACCESSION JO230548 VERSION JO230548.1 DBLINK BioProject: PRJNA68813 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Penium margaritaceum ORGANISM Penium margaritaceum Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae; Desmidiales; Peniaceae; Penium. REFERENCE 1 (bases 1 to 281) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 281) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..281 /organism="Penium margaritaceum" /mol_type="mRNA" /strain="SKD2004_CL18" /db_xref="taxon:102169" BASE COUNT 72 a 65 c 86 g 58 t ORIGIN 1 agcattagag gcagcttgtg cttctgctgc tgctccgcct tgtttccttt gattcagtcc 61 caaccacggg agtcaattcc gagaagattc ctgtcgacag gggacgacgc cttgctcgcg 121 cagcctcgat agagaaaacg gaattgctga tggggacggc gagttcagag tcacacaggc 181 agtggattgc cagacaagaa tggagagatg atgcgggaag gaatttgcgg ccagacgaac 241 gagttgcaca tggacggaaa aactagatga cttatcccgg g //