LOCUS       JO228888                 745 bp    mRNA    linear   TSA 23-FEB-2012
DEFINITION  TSA: Penium margaritaceum pmar_71836_c33590_c mRNA sequence.
ACCESSION   JO228888
VERSION     JO228888.1
DBLINK      BioProject: PRJNA68813
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Penium margaritaceum
  ORGANISM  Penium margaritaceum
            Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae;
            Zygnematophycidae; Desmidiales; Peniaceae; Penium.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F.
  TITLE     Broad phylogenomic sampling and the sister lineage of land plants
  JOURNAL   PLoS ONE 7 (1), E29696 (2012)
   PUBMED   22253761
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  AUTHORS   Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2011) Cell Biology and Molecular Genetics,
            University of Maryland, Bioscience Research Building, College Park,
            MD 20947, USA
COMMENT     Two-step clustering process: First, trimmed 454 reads were
            clustered with MIRA v.2.9.43 using 454 EST assembly specific
            parameters. Second, the raw Sanger reads were combined with the 454
            contigs + singleons (with quality scores) and were cleaned then
            clustered with CAP3 (100 bp plus 95 percent identity overlap).
            
            ##Assembly-Data-START##
            Assembly Method       :: MIRA v. 2.9.43; CAP3
            Sequencing Technology :: 454 GS FLX Titanium; Sanger
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Penium margaritaceum"
                     /mol_type="mRNA"
                     /strain="SKD2004_CL18"
                     /db_xref="taxon:102169"
BASE COUNT          107 a          259 c          227 g          144 t
ORIGIN      
        1 tgcgggcgtc cgcggaggtt gagcagacgg gggatggaag gcccgtacac gtcagcgtcg
       61 agaatgccca cttgcaggcc acacccgtgc gcaagtgccg ctgcgatgtt cacagctgta
      121 gttgactttc cgacgccacc cttgccagac gccacggcga tgacgtggcg caccccatcc
      181 ggcttcatgg ccttctgtcc cacagctggt gtgccgctgc cgcccgccga ccttgctggt
      241 gctccgctac ctgtggagaa cctcctcctg ctgctgctgc tgctgctgct gctgctgctg
      301 ctgccgccrt ccgccgccac cgccgctgct rctgctgccg csaccgccgc caaagatcgc
      361 caaccctggc cgaatcccct tacggcrgtt cgatgaggag cagctgcgsc gccraccagt
      421 tcagtagacg cgacttcttc cytgctgygg atgctcgcaa ccaagccgct gaatccgccg
      481 gtgtgctttg agtcaccgcc agctggcagt gcgtcaccgc cagctggcag tgagtcacag
      541 ccagctggca ggtgagtcac cgccagcttg tccatgcagt ggatgcgggt gcggcgacgg
      601 ggaacacttc ggctccgaat agactcgcgc tgatggtgct accgttgcac ctgccgctgc
      661 cgttgaggcg tggacgcgcg aacagtaggc tcctcgttcg gccgctctgc ttcgaactgc
      721 tgctgctgct gctgctgctg ccgca
//