LOCUS JO228368 460 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Penium margaritaceum pmar_71836_c32177_c mRNA sequence. ACCESSION JO228368 VERSION JO228368.1 DBLINK BioProject: PRJNA68813 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Penium margaritaceum ORGANISM Penium margaritaceum Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae; Desmidiales; Peniaceae; Penium. REFERENCE 1 (bases 1 to 460) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 460) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..460 /organism="Penium margaritaceum" /mol_type="mRNA" /strain="SKD2004_CL18" /db_xref="taxon:102169" BASE COUNT 102 a 104 c 171 g 79 t ORIGIN 1 agcgctgctg cttctgtagc agcagcagga gcagcagcgg cggctgcagc agcagcctct 61 gggcaaagcg gcggtggggg gagcaccact ggcgcagcag gtgctccttc acgggggssa 121 ctcaagtttg ggtttgggag gcggaggtgg gaggccagca agggcaagat ggcattcaac 181 gctgtgggaa agaagggtgg gcgtcctctc gtgcgaaacc gtcaaagcct gtggcatcca 241 ttttttggag atgctggaag cgacgacgaa gacgactgag ccgagagagc agctgcaacg 301 gcaggaaaat gggatgccaa gctggcgaaa gcagctgcaa gggcaggacc ttgggacgca 361 gagcaggtga cacagcttgc tgagtttcca tactctgttg catggccgac agattgtgct 421 ttgngcgttc aatgggattg taagngtgag caccggcggg //