LOCUS JO228302 391 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Penium margaritaceum pmar_71836_c32017_c mRNA sequence. ACCESSION JO228302 VERSION JO228302.1 DBLINK BioProject: PRJNA68813 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Penium margaritaceum ORGANISM Penium margaritaceum Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae; Desmidiales; Peniaceae; Penium. REFERENCE 1 (bases 1 to 391) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 391) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..391 /organism="Penium margaritaceum" /mol_type="mRNA" /strain="SKD2004_CL18" /db_xref="taxon:102169" BASE COUNT 101 a 138 c 68 g 84 t ORIGIN 1 gtcatcgtca ctcgtcactc gtcactcgtc actcgtcact cgtcactcgt catcgtcatc 61 gtcatcgtca tcgtcatcgt catcgtcatc atcaccacca ccaccgtcat caccagcaca 121 atcaccacca ctaccactac caccagcact agcatcagca ccagctgtag caaaaccacc 181 accacttcca ccatcatcta cattgttgac gtcaacaatg tcggcactac aactgtcaac 241 atcatctgtg gtggttaagt catgtgcaaa gttccccact cggcggcctg aagttgtgag 301 gtggcagagg tgcatcctca gcgcagacat ggaagagaca gcgtcctccc tttccctggc 361 acaaccaaaa cagatgcaaa catctccagc c //