LOCUS JO228297 491 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Penium margaritaceum pmar_71836_c32006_c mRNA sequence. ACCESSION JO228297 VERSION JO228297.1 DBLINK BioProject: PRJNA68813 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Penium margaritaceum ORGANISM Penium margaritaceum Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae; Desmidiales; Peniaceae; Penium. REFERENCE 1 (bases 1 to 491) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 491) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..491 /organism="Penium margaritaceum" /mol_type="mRNA" /strain="SKD2004_CL18" /db_xref="taxon:102169" BASE COUNT 122 a 119 c 150 g 100 t ORIGIN 1 tgctcgtgag ggggcctccg ggtctggcct ggctgaaggg cagcgctgga ggagggcttc 61 ctcgacgccg caaggtgacc aagaccaagg tgaagagccg atggggcgga gtggttggag 121 caggcgcgca ccaccaagtg tgaatgtgtg ggggaaggca aacacacgtc acaccacgcc 181 atgcatgaca tggcaccacg gctcggtaca acgttcacgt gacagggttg gagacaacgc 241 cacgcacaag tggtccattg gcgttcttag attgtctggg agtttcattg tttttttatg 301 taatgcagtt taagcgttgc tcacaaattg aatagatgtt gtgaccacag atcggcacag 361 cctagtacat gaaccaccca acaccgcgga agaaaatatg gaattacatc tcacgcaact 421 aatcctaatc ccacagacgg tgatagcgga ggtctggtgt cccatgatgg tacttccgcg 481 aggcagtgga g //