LOCUS JO227845 617 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Penium margaritaceum pmar_71836_c31057_c mRNA sequence. ACCESSION JO227845 VERSION JO227845.1 DBLINK BioProject: PRJNA68813 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Penium margaritaceum ORGANISM Penium margaritaceum Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae; Desmidiales; Peniaceae; Penium. REFERENCE 1 (bases 1 to 617) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 617) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..617 /organism="Penium margaritaceum" /mol_type="mRNA" /strain="SKD2004_CL18" /db_xref="taxon:102169" BASE COUNT 119 a 154 c 179 g 165 t ORIGIN 1 ccactcttgc cctctcaccc caaccccaga ctccctggct gtcacggagg cgcctcgcgt 61 tgcataaaca catgcatgtg cactcccttt tcgatgcttg aaacggagtg gtgcggtggg 121 tgtgtttgca gatgggagct tttccagatg tggagcattg gatagcacca aggatcgcgt 181 ttgtggctgc cgtggtactg acctgtttgc cgagcgcagt ttggagcttg aggacagcca 241 gaaggaggac cagggcgcga aggcatgggg ttcacgggcc ctcgactctc atgcctgtgt 301 ttgcacagtt cctgcaggcg catgctacac acttaggtgt gaagcacagt tagcaccatg 361 cttggttctg cggcgtagac ccatgcatgg atgggtacag accatgcctg aggcagcctg 421 atggcggtgc ctagcgagcc attctcacgc atggcatgcg tgcatgtcgg cccaaaattc 481 ggaatcaggg actgcttatt tatctgctgt gtatgtgaat gatggtcgtt gaagacttcg 541 tcaattttga tagcccctgg ggttggttgt ttgtagcttt cactatttct ttacctatgt 601 taacaactga ctgtttg //