LOCUS JO227771 704 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Penium margaritaceum pmar_71836_c30888_c mRNA sequence. ACCESSION JO227771 VERSION JO227771.1 DBLINK BioProject: PRJNA68813 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Penium margaritaceum ORGANISM Penium margaritaceum Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae; Desmidiales; Peniaceae; Penium. REFERENCE 1 (bases 1 to 704) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 704) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..704 /organism="Penium margaritaceum" /mol_type="mRNA" /strain="SKD2004_CL18" /db_xref="taxon:102169" BASE COUNT 138 a 186 c 270 g 110 t ORIGIN 1 caatgcaaca ccgcggtacg gaccaaagtt ccatgcaccc tagtcttcac ccaacgacgc 61 cggtcgcaaa gttgacgcgc ttcacatcgg acccagcact gtggtattgg aacccaggca 121 cgggcgtctg cgcaaactgt gcggcagtga gggcggggcc cttggcgaag gtgacgcact 181 ggcggccagt ggtggtgagc tgaacgccga agcgcatggc cacggtggag ccgtcaggca 241 cctccactgt ggggctgccc accgtgaacg cattggtggg cgtcgccagg gccttcccca 301 tgacatagga cttggcaatg aaggggcgaa cggcgatctg cgctggattg gcgccgttgg 361 cgtagcccat gaggacctgc gagttgagca tggcacagga gggggagagg ccaaacccaa 421 tccacccgta ggattcggag gtggaggcac gcacgatggg cattggctgt cttgccagag 481 agggtccagg agagggtcac gccggaacca aggttcacgc agaaggactg gggcacggtg 541 acgccaccga aggtgtctgt gcaacccccc gccgggggcg gagacttggg cgtcaagggc 601 ggtgacttgg ggcttgcagg cggggacctg agaggggggc tccctaggag ggggattttg 661 gggaggggga ggctctttgg gggggaggat tttggcagga gacg //