LOCUS JO217376 495 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Penium margaritaceum pmar_71836_c13050_c mRNA sequence. ACCESSION JO217376 VERSION JO217376.1 DBLINK BioProject: PRJNA68813 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Penium margaritaceum ORGANISM Penium margaritaceum Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae; Desmidiales; Peniaceae; Penium. REFERENCE 1 (bases 1 to 495) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 495) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..495 /organism="Penium margaritaceum" /mol_type="mRNA" /strain="SKD2004_CL18" /db_xref="taxon:102169" BASE COUNT 109 a 121 c 135 g 130 t ORIGIN 1 ggagagtatg actgcgacat ttcactagag cggctcgtca tccagaacgt cttcggcaat 61 gtacaattgg gcgcttgctc actgtccgca cgatacgtcg tcttccgcaa caatgtgggc 121 ccaaagggcg ttcttgcaac actcgactac tcgtcctcct atactggccc tgacatgttc 181 ttccgctgtg attttgtcaa caacactgcg ctgtttggaa atgggggtgt gttcaacttt 241 ggcaatccca acgggatgat tcagaccact ctgagcttct acaactgcac ctttcgcaac 301 aaccgcgcgc tcagcggcat gggcggtgtg atctacggtg ccggaagtaa catgatcttg 361 caaaactctg tctttgaggg aaactcagca ggtctgttgc atgcactcgg aggatgcttc 421 cagagagggt gcgggaaact gtgagaattt tgggagaggt tatgtgtcca tgatcttgtt 481 ttatgggaga cagtg //