LOCUS JO216722 571 bp mRNA linear TSA 11-JAN-2012 DEFINITION TSA: Penium margaritaceum pmar_71836_c11991_c mRNA sequence. ACCESSION JO216722 VERSION JO216722.1 DBLINK BioProject: PRJNA68813 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Penium margaritaceum ORGANISM Penium margaritaceum Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae; Desmidiales; Peniaceae; Penium. REFERENCE 1 (bases 1 to 571) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 571) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..571 /organism="Penium margaritaceum" /mol_type="mRNA" /strain="SKD2004_CL18" /db_xref="taxon:102169" BASE COUNT 106 a 178 c 166 g 114 t ORIGIN 1 agcagcacag ctgcaggcct gttagtccca tctggcgtgt cgatggcagc acctccttgg 61 agaccacccg ccttcacaaa ggccgcggca ccttcctcca aagctatcga ctgcagcaac 121 agactcagca gcttgtgctg ccgccacgtc agacgacgcg gctttctcca ctgctgcaac 181 agtttctggg tttgcgggca gcaggcgcga tgcgtcagta gatcctagac acccagcagg 241 gcacggtgga ccagaaggca tcgcagaagg agcggaatcg gctggctcga gaggagcgtc 301 ggcgactcca gcagccctaa gcggacctgc aagcgacccc ggcgtgtcgg aagcaggcac 361 ttgtgcctca gccgaagaag gagatgcagt ccggccggat gttccagatc tgctactctt 421 gtctagtcct gtttgtggtg ttgacgagcg catggcagtc gccgygtcty cttcagcctc 481 gcaggcttga ggctkctcac gcccttcaac ctgstgaacr wcgcacatat cctcytcctg 541 gtcctcagtc gtttgcttgc gcggctgttg t //