LOCUS JO216076 504 bp mRNA linear TSA 11-JAN-2012 DEFINITION TSA: Penium margaritaceum pmar_71836_c11068_c mRNA sequence. ACCESSION JO216076 VERSION JO216076.1 DBLINK BioProject: PRJNA68813 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Penium margaritaceum ORGANISM Penium margaritaceum Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae; Desmidiales; Peniaceae; Penium. REFERENCE 1 (bases 1 to 504) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 504) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..504 /organism="Penium margaritaceum" /mol_type="mRNA" /strain="SKD2004_CL18" /db_xref="taxon:102169" BASE COUNT 89 a 160 c 118 g 137 t ORIGIN 1 catccatgcg gccatcgtct ctgcccaggt ccgtgggacc cgatcggcca tcagcatcat 61 gtttttggca tctctgccca agtctcgatc ttcctcgcca tcattgctgg agcaatcaat 121 cgattcctct ttgtcgtcat tgtcattatc gtcgtcatca tcatcctccg cctcatccct 181 cgaatcatcg ccgcccgaat catcatgcga aggaccggag gaggtttttc tggccatcaa 241 gtttgagaac atggccatga tgcactgccc tggcagctac cgtgccaaag cgctgttcca 301 tagcgcatgc ccttccactt gactcgccgg ttcagctccc gcagtgtgtg ctttctttat 361 cgctcgagac ggcgtgggtc gccgaggcaa actccgtctt ggcgatcaat gcgactgagc 421 gttgtatggc acaaagtgca tcgtggaggg tgcaccacgt ccttgcctga tcttctcttg 481 taaccttcat gacctcttcc ttgc //