LOCUS JO215733 622 bp mRNA linear TSA 11-JAN-2012 DEFINITION TSA: Penium margaritaceum pmar_71836_c10567_c mRNA sequence. ACCESSION JO215733 VERSION JO215733.1 DBLINK BioProject: PRJNA68813 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Penium margaritaceum ORGANISM Penium margaritaceum Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae; Desmidiales; Peniaceae; Penium. REFERENCE 1 (bases 1 to 622) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 622) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..622 /organism="Penium margaritaceum" /mol_type="mRNA" /strain="SKD2004_CL18" /db_xref="taxon:102169" BASE COUNT 161 a 146 c 165 g 150 t ORIGIN 1 tccgcctcct ccttctccac caacgcttcc ttcatttaag ccttcgctga gaccatggct 61 ttccgcatcg tgctcatctc ggctctgatc ctcctctccg tctttgaagg cgttgacgct 121 cacttcccga ggaagctaac tgccattgag gacagctcga gcaccaaagt ggttcggtat 181 gccaaggacg gcaagaaggt ggagaacgct atcgaggctg gagacactta cctgtggacg 241 gggagcatca tcaacacggt caaaaagact aagattggat cggactatgg caactgcctt 301 tactcagcca agggttattc catctgcacc tacacaatca atctgaagag aggaagcatc 361 acagtgactg gtacttatga agacgcaaag gcagtgcaaa ttcaatctgt tgttggtgga 421 actgggctct atgctggggc aaagggatat gtgagggcca gggcctttgg tgacaactac 481 aagtatgagg tcttcctcat atagagatgt tgggcatgac ctagaaactg cgatgctgag 541 ggaatgatat tgagaaagaa gaggcgagtc tctggtgaat gatgaactct ggtggaaata 601 aagaagggtc tttctttctc cc //