LOCUS JO215513 970 bp mRNA linear TSA 11-JAN-2012 DEFINITION TSA: Penium margaritaceum pmar_71836_c10228_c mRNA sequence. ACCESSION JO215513 VERSION JO215513.1 DBLINK BioProject: PRJNA68813 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Penium margaritaceum ORGANISM Penium margaritaceum Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae; Desmidiales; Peniaceae; Penium. REFERENCE 1 (bases 1 to 970) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 970) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..970 /organism="Penium margaritaceum" /mol_type="mRNA" /strain="SKD2004_CL18" /db_xref="taxon:102169" BASE COUNT 180 a 342 c 203 g 244 t ORIGIN 1 cttttgttaa gcttacttaa tcatgtacnc tagaaaaaaa tacagaaaga gccatgcttt 61 acggttccga aaagaagaga atattctgca cactctcctt atttgccaga aaagtcttca 121 gcacgtcggt tatcgagaga ggagaagccc tccgtgcgat gcaccacgcc tgcaggtgct 181 ctgcttcctc ccccgtgctt ctcccatgcc agtagcgctg ccgtctcctc acgctcctcc 241 tcctcccctt cctccttcct cccctcttcc tcctccccct cctcattcac ctttccagcc 301 ttctcttcct catcagcatc aacggtatct ttcaacagcc aggccgcttc cctctcctgc 361 ttctggcatg gtgcccacac gtccgacatc tcctcctcct tgtcctcctc gtcggccttg 421 tccttctcct catcctcctc caccacgctc ctccactcct ccgcttccct gctgtctgta 481 agggccttga ggtacaagtc gccggcgagc ttggcactca gtttggcatg gagcttggag 541 tcgatcgcgg agtaaagatt gtggtccttg ttggcgagca agtagactgc caacatcacc 601 cgctgcacta ggagtccatt ctcatccttt ttgaagaaag ggcggaacga ttccgtcgta 661 aacacaaact ccgacccccc ctccttgtgc acggccgggc ggtcaaagtg gcccgatgga 721 aaatgttttg tgcgcattgt ctgcgcgtcg tagccttcgc tgtagcccga tagccattgc 781 agcatttcct ggcaccgcgt aaacctttac gcctaggtcg accttcctgg caccgcgtaa 841 acccgtgcgc taggtcgcca tacctggcac cgcgtaaacc cgtacgctag gtcgccacac 901 ttgcgcttgc actccaccac ggccaccacc ctccaccact ttgaaacgcc gaggcttcat 961 ttggccatgg //