LOCUS       JK034174                 875 bp    mRNA    linear   EST 07-FEB-2014
DEFINITION  92 (LHA-2011) Bellamya aeruginosa normalized cDNA library Bellamya
            aeruginosa cDNA 5', mRNA sequence.
ACCESSION   JK034174
VERSION     JK034174.1
DBLINK      BioSample: SAMN00622379
KEYWORDS    EST.
SOURCE      Sinotaia aeruginosa
  ORGANISM  Sinotaia aeruginosa
            Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
            Caenogastropoda; Architaenioglossa; Viviparoidea; Viviparidae;
            Sinotaia.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   An,L.H. and Zheng,B.H.
  TITLE     Construction and Characterization of Normalized cDNA Library from
            the Snail Bellamya aeruginosa Exposure to Copper
  JOURNAL   Unpublished
COMMENT     Contact: An L.H.
            State Environmental Protection Key Laboratory Of Estuarine And
            Coastal Research
            Chinese Research Academy For Environment Sciences
            8 Dayangfang BeiYuan Road, Chaoyang District, Beijing 100012, China
            Tel: +86-10-84913914
            Fax: +86-10-84913914
            Email: anlh@craes.org.cn
            PCR PRimers
            FORWARD: CTCGGGAAGCGCGCCATTGTGTTGGT
            BACKWARD: ATACGTCTCACTATAGGGCGAATTGGCC.
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Sinotaia aeruginosa"
                     /mol_type="mRNA"
                     /db_xref="taxon:2653872"
                     /tissue_type="gonads; digestive gland; peripheral tissue"
                     /clone_lib="SAMN00622379 Bellamya aeruginosa normalized
                     cDNA library"
                     /dev_stage="mature"
                     /note="Vector: Lambda TripEx2; 1.5 microliter RNA solution
                     (1-3 microgram of total RNA) was normalized using CDS and
                     SMART Oligo IV primer firstly, and then the reaction
                     solution was reversed transcription for synthesizing the
                     first-strand using MMLV reverse transcriptase (Promega,
                     USA). Subsequently, PCR reaction was operated and cDNA was
                     extracted using Promegas PCR clean-up system kit (Madison,
                     USA). And then the cDNA library was constructed using
                     SMART cDNA Library Construction Kit. After that, 6000
                     random clones were sequenced by the ABI3730xl. BLAST
                     homology searches in the nr and nt databases in GenBank
                     were performed for each of the unigenes under Evalues of
                     1e-5. The unigenes were named after the homologous
                     sequences in GenBank. The annotated unigenes were then
                     matched to specific processes or functions using COG and
                     GO tools."
BASE COUNT          224 a          173 c          254 g          224 t
ORIGIN      
        1 tggagtgtaa gaattacaac aagcttggaa caggatatgg tcattacaca ggaacagacg
       61 caggtgttat ttgcggagat cacgttaagt catcctttcc tgtacgcctt gttaacggat
      121 ctagtccgtg ggaaggtcgc gttgaattgc agtcgaatgg tcagtggggc acgatatatt
      181 acagcggctg ggatgctgat gacgcaagag ttatctgccg tatgctaggt ttcaacagtt
      241 atgcaatccc cgtaacttca aaccagtttg gagtgggtac aggcccggtt tatctgtatg
      301 gtatgcgatg tgatgggacg gagaaacata ttcttgaatg ccccagtaac gaccaatttg
      361 gagttggcag ctatggtcgt agtattgatg ccggtgtcat ctgtggtaac gggactcttt
      421 caaactatcc agttcgtctt gtaaacgggt caggaccctg ggagggacga gttgagattc
      481 aagcctttgg acattgggga acggtgtcct catccaactg ggacactaat gaagctagag
      541 ttgtttgtca tatgctgggt tataatagtt acgcaacagc ccttacagtg gcagcaaacc
      601 atttcggtag cgggacgggt ccagtctggc tgaacaatat ccagtgtgat ggaacagaga
      661 cccatctgct tgagtgcccg agtactaata tgatgaattc tggcggcggg acagtgaccc
      721 attcctcaga cgctggtgtc gtatgtggga acggaagtct ctctatgtac cccgttagat
      781 tagtgaacgg aaattcgaca cgagaaggac ggatagaagt acaagtgaac ggggtatggg
      841 gcacggtgtg tgacgacaac tgggactcta atgat
//